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(12)United States Patent
Matsumoto |
(10) Patent No.:
US6,177,106 B1
(45) Date of Patent:
Jan. 23, 2001 |
|
| (54) |
METHOD FOR FRACTIONATING RED BLOOD CELLS AND ANTIBACTERIAL MATERIALS FOR BACTERIAL
PROLIFERATION INHIBITORS PRODUCED THEREBY |
(52) |
U.S. CI............
424/533; 424/529; 435/2 |
| (58) |
Field of Search........
435/2; 424/529, 424/533 |
| Primary Examiner-Sandra E Saucier |
| (74) |
Attorney, Agent, or Firm-Liniak, Berenato, Longarce & White, LLC |
| (57) |
ABSTRACT |
| (76) |
Inventor:
Tsukasa Matsumoto,
803 Estecion Oomori,
2-1-20,
Oomorinaka, Oota-ku,
Tokyo 143-0014 (JP) |
A method for fractonationg red
blood
cells of human blood into there
fractions
comprises following steps;
(a) human blood sample is mixed with
dextran aqueous solution and
maintainedstationarily for 60 to 75 min
to
fractionate
this blood sample
into three
layers ;
(b) the upper, intermediate, and lower layer
samples are individually separated and
collected; and
(c) the upper layer sample is treated with
hypotonic solution for a short period
and
then added with hypertonic solution.
Further the invention provides
antibacterial or bacterial proliferation
inhibitory material produced by the
method. This method can easily
fractionate three different blood fractions
including red blood cells as a main
component and having different functions.
These three different fractions can be
applied to several clinical tests such as
antibacterial test and the like. |
| (*) |
Notice:
Under 35 U.S.C 154(b), the term of this patent shall be extended for 0 days. |
| (21) |
Appl. No.:
09/138,892 |
| (22) |
Filed:
Aug. 24, 1998
Related U.S.
Application Date |
| (63) |
Continuation-in-part of applicaton No. 08/803,458, filed on Feb. 20, 1997, now abandoned. |
| (30) |
Foreign application Priority Date
Aug. 15 1996 (JP).....8-215552 |
| (51) |
Int. CI.7 ...... A61K 35/18; A61K 35/14 ;A0IN 1/02 |
21 Claims,34 Drawing Sheets |
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